Glutathion-S-transferase

Finished

Glutathione transferases comprise a large class of multifunctional enzymes, some involved in detoxification pathways. Since these enzymes are able to interact with potentially toxic molecules, they could be used as targets to screen for compounds with biological activity.

To test this hypothesis, glutathione transferases (GSTs) from the white-rot fungus Trametes versicolor have been used to screen for antifungal molecules from a library of tropical wood extracts.

The interactions between a set of six GSTs from the omega class and 116 extracts from 21 tropical species were quantified using a high-throughput thermal shift assay. A correlation between these interactions and the antifungal properties of the tested extracts was demonstrated. This approach has been extended to the fractionation of an Andira coriacea extract and led to the detection of maackiain and lapachol in this wood. Altogether, the present results supported the hypothesis that such detoxification enzymes could be used to detect biologically active molecules.

see :

• Barbier, M., Perrot, T., Salzet, G., Amusant, N., Dumarçay, S., Gérardin, P., Morel-Rouhier, M., Sormani, R., & Gelhaye, E. (2020a). Glutathione Transferases: Surrogate Targets for Discovering Biologically Active Compounds. Journal of Natural Products, 83(10), 2960–2966. https://doi.org/10.1021/acs.jnatprod.0c00480

• Barbier, M., Perrot, T., Salzet, G., Amusant, N., Dumarçay, S., Gérardin, P., Morel-Rouhier, M., Sormani, R., & Gelhaye, E. (2020b). Glutathione Transferases: Surrogate Targets for Discovering Biologically Active Compounds. Journal of Natural Products, 83(10), 2960–2966. https://doi.org/10.1021/acs.jnatprod.0c00480

• Perrot, T., Salzet, G., Amusant, N., Beauchêne, J., & Gelhaye, É. (2020, May 12). From fungal detoxification systems to wood durability in neotropical forests. 50th RWP Annual Meeting,. https://hal.science/hal-03145384

• Perrot, T., Salzet, G., Amusant, N., Beauchene, J., Gérardin, P., Dumarçay, S., Sormani, R., Morel‐Rouhier, M., & Gelhaye, E. (2020a). A reverse chemical ecology approach to explore wood natural durability. Microbial Biotechnology, 13(5), 1673–1677. https://doi.org/10.1111/1751-7915.13540

• Perrot, T., Salzet, G., Amusant, N., Beauchene, J., Gérardin, P., Dumarçay, S., Sormani, R., Morel‐Rouhier, M., & Gelhaye, E. (2020b). A reverse chemical ecology approach to explore wood natural durability. Microbial Biotechnology, 13(5), 1673–1677. https://doi.org/10.1111/1751-7915.13540

• Perrot, T., Schwartz, M., Saiag, F., Salzet, G., Dumarçay, S., Favier, F., Gérardin, P., Girardet, J.-M., Sormani, R., Morel-Rouhier, M., Amusant, N., Didierjean, C., & Gelhaye, E. (2018). Fungal Glutathione Transferases as Tools to Explore the Chemical Diversity of Amazonian Wood Extractives. ACS Sustainable Chemistry & Engineering, 6(10), 13078–13085. https://doi.org/10.1021/acssuschemeng.8b02636